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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Bovine Milk-Derived Exosomes as a Drug Delivery Vehicle for miRNA-Based Therapy
doi: 10.3390/ijms22031105
Figure Lengend Snippet: ( A ) Western Blot analysis of proteins present (Hsp90, CD63 and TSG101) or absent (calnexin) in exosomes and abundant in bovine milk (β-casein). Protein evaluation in: bovine skimmed milk (SM); exosomes obtained from SM by one (1U) or two (2U) ultracentrifugation steps; and the cellular fraction (CF). Equal amount of protein was loaded. Elution protein profile (F.1, F.8 to F.40) of bovine exosomes isolated by 1U followed by SEC (1U + SEC) ( B ) or 2U + SEC ( C ). Protein concentration (mg/mL) was estimated by the BCA assay. WB of SEC elution fractions from exosomes isolated by 1U + SEC ( D ) or 2U + SEC ( E ). Evaluation of Hsp90, CD63, TSG101, Calnexin and β-casein levels in each fraction (F.1, F.8 to F.40). Mimic hsa-miRNA-148a-3p elution profile (relative expression) of exosomes isolated from skimmed milk by U + T + SEC ( F ) or U + T + U + SEC ( G ). Mw: Molecular weight marker (Bio-Rad).
Article Snippet: Membranes were then incubated with appropriate primary antibodies: anti-Hsp90 (610418, BD, Madrid, Spain),
Techniques: Western Blot, Isolation, Protein Concentration, BIA-KA, Expressing, Molecular Weight, Marker